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Viser: Principles and Reactions of Protein Extraction Purification and Characterization

Principles and Reactions of Protein Extraction Purification and Characterization, 2. udgave

Principles and Reactions of Protein Extraction Purification and Characterization

Hafiz Ahmed
(2017)
Sprog: Engelsk
Taylor & Francis Group
1.818,00 kr.
Denne bog er endnu ikke udgivet. Den forventes Jul 2017.
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Detaljer om varen

  • 2. Udgave
  • Paperback: 544 sider
  • Udgiver: Taylor & Francis Group (Juli 2017)
  • ISBN: 9781439838396

This book describes the principles and reactions for most techniques of protein extraction, purification, and characterization. The second edition covers modern techniques that are extensively used in protein identification and analyses including NMR, mass spectrometry, recombinant protein, and database search. It incorporates NMR and mass spectrometry for protein identification, use of computers and websites for protein annotation, and identification of commonly modified proteins. The text also expands coverage of the recombinant proteins. Two new chapters address the identification and annotation of the target protein and the identification of other posttranslational modifications.

Extraction of Protein Preparation of Buffers for Protein Extraction Use of Protease Inhibitors in Extraction Use of Detergents in Extraction Chemical Lysis for Protein Extraction Mechanical Lysis for Protein Extraction Preparation of Extracts from Prokaryotes Preparation of Extracts from Yeast Preparation of Extracts from Eukaryotes Preparation of Extracts from Plants Preparation of Membrane Extracts Preparation and Extraction of Recombinant Protein Take-Home Message References Estimation of Protein Ultraviolet Absorption Methods Colorimetric Methods Fluorescent Methods Take-Home Message References Electrophoretic Analyses of Protein Driving Force of Electrophoresis Polyacrylamide Gel Electrophoresis
3.
3 Isoelectric Focusing Two-Dimensional (2D) Gel Electrophoresis Western Blotting Capillary Electrophoresis Take-Home Message References Purification of Protein General Consideration and Purification Strategy Non-Chromatographic Purification of Proteins Chromatographic Purification of Proteins Take-Home Message References Antibodies: Structures, Interactions, and Production Immune System and Antibody Response Structure of Antibodies Antigen-Antibody Interactions Production of Antibodies Development of Monoclonal Antibodies Purification of Antibodies Take-Home Message References Antibody Labeling, Antibody Detection, and Immunochemical Techniques Methods of Labeling Antibodies Detection of Antibody Binding Inununochemical Techniques: A Wide Range ofInununoassays for Detecting and Quantitating (Semi) Antigen-Antibody Reactions Immunoprecipitation Immunohistochemistry Flow Cytometry Take-Home Message References Identification and Annotation of the Target Protein Enzymatic Cleavage of Protein Chemical Cleavage Sequencing Peptides Identification of Protein by Mass Spectrometry Annotation by Protein Database Search Take-Home Message References Identification of Most Common Post-translational Modification (Glycosylation): Purification of Glycoproteins and Analyses of Their Oligosaccharides Some Basics of Carbohydrates Is My Protein Glycosylated? Purification of Glycoproteins Analyses of Carbohydrate Structures Take-Home Message References Identification of Other Posttranslational Modifications Phosphorylation Palmitoylation Myristoylation GPI Modification Acetylation Methylation Transamidation Ubiquitination Hydroxylation of Proline and Lysine Proteolytic Degradation Take-Home Message References
Index
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