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Viser: Plant Biotechnology and Genetics - Principles, Techniques, and Applications
Plant Biotechnology and Genetics
Principles, Techniques, and Applications
C. Neal Stewart
(2025)
Sprog: Engelsk
John Wiley & Sons, Limited
1.487,00 kr.
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Detaljer om varen
- 3. Udgave
- Hardback: 512 sider
- Udgiver: John Wiley & Sons, Limited (Marts 2025)
- ISBN: 9781394217212
Discover the latest edition of this authoritative textbook on plant biotechnology and genetics
Plant biotechnology is a field of research and development in which scientific techniques are brought to bear on the creation and modification of new, beneficial plants and strains. Biotechnological techniques can be used to add nutritive value, increase resistance to diseases and pests, increase yields, and more. The production of biotech crops has increased over one hundred times since their introduction into commercial agriculture in 1996, making them the most rapidly-adopted crop category in the history of modern agriculture.
Plant Biotechnology and Genetics is the essential introduction to this thriving research subject. Beginning with an overview of basic plant biology and genetics, it then moves to the fundamental elements of biotechnology. Now fully updated to reflect the latest research advances and technological breakthroughs, it continues to be a must-own for readers interested in the future of food production and more.
Readers of the third edition of Plant Biotechnology and Genetics will also find:
- New chapters covering topics like genome editing, chloroplast genome engineering, and synthetic biology
- Updates throughout to incorporate increased coverage of haploid production, genomic selection, and more
- Summary and discussion questions in each chapter, along with a companion website incorporating images and lecture materials
Plant Biotechnology and Genetics is ideal for advanced undergraduate and masters students in plant biotechnology courses, as well as professionals seeking a helpful reference guide.
List of Contributors xv Preface xvii
1. The Impact of Biotechnology on Plant Agriculture 1 Graham Brookes
1.0.
Chapter Summary and Objectives 1
1.1. Introduction 1
1.2. Cultivation of Biotechnology (GM) Crops 2
1.3. Why Farmers Use Biotech Crops 4
1.4. GM Crop Trait Use on Production and Farming 6
1.5. How the Adoption of Plant Biotechnology has Impacted the Environment 7
1.6. Conclusions 13 References 15
2. Mendelian Genetics and Plant Reproduction 17 Matthew D. Halfhill and Suzanne I. Warwick
2.0.
Chapter Summary and Objectives 17
2.1. Genetics Overview 17
2.2. Mendelian Genetics 20
2.3. Mitosis and Meiosis 24
2.4. Plant Reproductive Biology 28
2.5. Conclusion 34 Life Box
2.1. Richard A. Dixon 35 Life Box
2.2. Michael L. Arnold 36 References 38
3. Plant Breeding 39 Nicholas A. Tinker and Elroy R. Cober
3.0.
Chapter Summary and Objectives 39
3.1. Introduction 40
3.2. Central Concepts in Plant Breeding 41
3.3. Objectives in Plant Breeding 52
3.4. Methods of Plant Breeding 53
3.5. Breeding Enhancements 64
3.6. Conclusions 71 Life Box
3.1. Gurdev Singh Khush 71 Life Box
3.2. P. Stephen Baenziger 73 References 75
4. Plant Development and Physiology 76 Glenda E. Gillaspy and Catherine P. Freed
4.0.
Chapter Summary and Objectives 76
4.1. Plant Anatomy and Morphology 77
4.2. Embryogenesis and Seed Germination 78
4.3. Meristems 84
4.4. Leaf Development 87
4.5. Flower Development 90
4.6. Hormone Physiology and Signal Transduction 93
4.7. Conclusions 99 Life Box
4.1. Natasha Raikhel 99 Life Box
4.2. Brenda S.J. Winkel 101 References 103
5. Tissue Culture: The Manipulation of Plant Development 105 Vinitha Cardoza
5.0.
Chapter Summary and Objectives 105
5.1. Introduction 105
5.2. History of Tissue Culture 106
5.3. Media and Culture Conditions 107
5.4. Sterile Technique 109
5.5. Culture Conditions and Vessels 111
5.6. Culture Types and Their Uses 113
5.7. Regeneration Methods of Plants in Culture 119
5.8. Rooting of Shoots 122
5.9. Acclimation 122
5.10. Automation in Plant Tissue Culture 123
5.11. Artificial Intelligence (AI) and Machine Learning in Plant Tissue Culture 123
5.12. Problems That Can Occur in Tissue Culture 123
5.13. Conclusions 124 Acknowledgments 124 Life Box
5.1. Vinitha Cardoza 125 Life Box
5.2. Raymond D. Shillito 126 References 129
6. Molecular Genetics of Gene Expression 133 Maria Gallo and Alison K. Flynn
6.0.
Chapter Summary and Objectives 133
6.1. The Gene 134
6.2. DNA Packaging into Eukaryotic Chromosomes 134
6.3. Transcription 135
6.4. Translation 144
6.5. Protein Postranslational Modification 150 Life Box
6.1. Maarten Chrispeels 150 Life Box
6.2. Hong S. Moon 152 References 153
7. Plant Systems Biology 155 Wusheng Liu, Yongil Yang, and C. Neal Stewart, Jr.
7.0.
Chapter Summary and Objectives 155
7.1. Introduction 156
7.2. Defining Plant Systems Biology 157
7.3. Properties of Plant Systems 158
7.4. A Framework of Plant Systems Biology 160
7.5. Disciplines and Enabling tools of Plant Systems Biology 162
7.6. Conclusions 179 Life Box
7.1. C. Robin Buell 180 Life Box
7.2. Joshua Yuan 182 References 183
8. Recombinant DNA, Vector Design, and Construction 185 Stephen L. Gasior, David G.J. Mann, and Mark D. Curtis
8.0.
Chapter Summary and Objectives 185
8.1. Plasmids are Unique Genetic Elements in Nature 186
8.2. DNA Vectors 189
8.3. Recombinant DNA Methods 195
8.4. Vector Design in Plant Research and Trait Development 206
8.5. Vectors for Targeted Genome Manipulations 213
8.6. Prospects 216 Life Box
8.1. David Mann 216 References 218
9. Genes and Traits of Interest 224 Joanna H. Kud and Kenneth L. Korth
9.0.
Chapter Summary and Objectives 224
9.1. Introduction 225
9.2. Identifying Genes of Interest Via Omics Technologies 225
9.3. Traits for Improved Crop Production Using Transgenics 228
9.4. Conclusion 245 Life Box
9.1. Tony Shelton 246 References 247
10. Promoters and Marker Genes 249 Wusheng Liu, Debao Huang, C. Neal Stewart, Jr., and Brian Miki
10.0.
Chapter Summary and Objectives 249
10.1. Introduction 250
10.2. Promoters 250
10.3. Marker Genes 259
10.4. Marker- Free Strategies 270
10.5. Conclusions 272 Life Box
10.1. Wusheng Liu 274 Life Box
10.2. Yunde Zhao 275 References 277
11. Transgenic Plant Production 282 John J. Finer and Ning Zhang
11.0.
Chapter Summary and Objectives 282
11.1. Overview of Plant Transformation 283
11.2. Agrobacterium Tumefaciens 286
11.3. Particle Bombardment 293
11.4. Other Methods of Transformation 297
11.5. The Rush to Publish 300
11.6. A Look to the Future 306 Life Box
11.1. John Finer 306 Life Box
11.2. Kan Wang 308 Life Box
11.3. Ted Klein 310 References 312
12. Analysis of Transgenic Plants 315 C. Neal Stewart, Jr.
12.0.
Chapter Summary and Objectives 315
12.1. Essential Elements of Transgenic Plant Analysis 316
12.2. Assays for Transgenicity, Insert Copy Number, and Segregation 317
12.3. Transgene Expression 323
12.4. Knockdown or Knockout Analysis Rather Than Overexpression Analysis 326
12.5. The Relationship Between Molecular Analyses and Phenotype 327 Life Box
12.1. Neal Stewart 327 Life Box
12.2. Nancy A. Reichert 329 References 331
13. Plastid Genetic Engineering 332 Alessandro Occhialini and Scott C. Lenaghan
13.0.
Chapter Summary and Objectives 332
13.1. Introduction 333
13.2. Plastid Biology and Molecular Genetics 334
13.3. Plastid Genetic Engineering History and Motivations 336
13.4. Plastome Engineering Versus Nuclear Genome Engineering 336
13.5. Key Components for Plastome Engineering of Plants 338
13.6. Plastome Transformation Vector Design 340
13.7. Beyond Transplastomics: The Use of Episomal Vectors for Minisynplastome and Minichromosome Approaches 347
13.8. Removing DNA from Plastids 349
13.9. The Future of Plastid Engineering 350 Life Box
13.1. Henry Daniell 351 Life Box
13.2. Pal Maliga 354 Life Box
13.3. Alessandro Occhialini 356 Life Box
13.4. Ralph Bock 357 References 358
14. CRISPR- Cas: Genome Editing from Small- Scale to High Throughput for Plant Biology and Biotechnology 366 S.P. Avinash, Mirza J. Baig, and Kutubuddin A. Molla
14.0.
Chapter Summary and Objectives 366
14.1. Introduction 367
14.2. Diverse CRISPR Approaches and Tools for Precisely Editing Genomes 371
14.3. Changing Gene Expression by CRISPR 374
14.4. CRISPR Screening for Large- Scale Functional Genomics 376
14.5. CRISPR- Enabled Crop Improvement 377
14.6. Commercialized Genome- Edited Crops 380
14.7. Conclusions 380 Life Box
14.1. Kutubuddin Molla 381 Life Box
14.2. Dan Voytas 382 Life Box
14.3. Yiping Qi 383 References 385
15. Regulations and Biosafety 390 Alan Mchughen and Stuart J. Smyth
15.0.
Chapter Summary and Objectives 390
15.1. Introduction 390
15.2. History of Genetic Engineering and its Regulation 392
15.3. Regulation of GE Plants 394
15.4. Regulatory Flaws and Invalid Assumptions 402
15.5. The State of Genome Editing Regulation 406
15.6. Conclusion 409 Life Box
14.1. Alan Mchughen 411 References 412
16. Field Testing of Transgenic Plants: Risk Assessment and Performance 415 Detlef Bartsch, Achim Gathmann, Arti Sinha, and Christiane Saeglitz
16.0.
Chapter Summary and Objectives 415
16.1. Introduction 416
16.2. Environmental Risk Assessment Process 416
16.3. An Example Risk Assessment: The Case of Bt Maize 418
16.4. Proof of Safety Versus Proof of Hazard 422
16.5. Modeling the Risk Effects on a Greater Scale 422
16.6. Proof of Benefits: Agronomic Performance 423
16.7. Conclusions 424 Life Box
18.1. Detlef Bartsch 426 References 427
17. Intellectual Property in Agricultural Biotechnology: Strategies for Open Access 429 Gregory Graff, David Jefferson, Monica Alandete-Saez, Cecilia Chi-Ham, Sara Boettiger, and Alan B. Bennett
17.0.
Chapter Summary and Objectives 429
17.1. Intellectual Property and Agricultural Biotechnology 430
17.2. The Relationship Between Intellectual Property and Agricultural Research 433
17.3. Patenting Plant Biotechnology: The Anti- Commons Problem 434
17.4. What Is Freedom to Operate? 438
17.5. Strategies for Open Access 441
17.6. Conclusions 443 Life Box
17.1. Alan Bennett 444 Life Box
17.2. Maud Hinchee 445 References 446
18. Why Transgenic Plants Are So Controversial 451 Jennifer Trumbo and Douglas Powell
18.0.
Chapter Summary and Objectives 451
18.1. Introduction 452
18.2. Perceptions of Risk 454
18.3. R
1. The Impact of Biotechnology on Plant Agriculture 1 Graham Brookes
1.0.
Chapter Summary and Objectives 1
1.1. Introduction 1
1.2. Cultivation of Biotechnology (GM) Crops 2
1.3. Why Farmers Use Biotech Crops 4
1.4. GM Crop Trait Use on Production and Farming 6
1.5. How the Adoption of Plant Biotechnology has Impacted the Environment 7
1.6. Conclusions 13 References 15
2. Mendelian Genetics and Plant Reproduction 17 Matthew D. Halfhill and Suzanne I. Warwick
2.0.
Chapter Summary and Objectives 17
2.1. Genetics Overview 17
2.2. Mendelian Genetics 20
2.3. Mitosis and Meiosis 24
2.4. Plant Reproductive Biology 28
2.5. Conclusion 34 Life Box
2.1. Richard A. Dixon 35 Life Box
2.2. Michael L. Arnold 36 References 38
3. Plant Breeding 39 Nicholas A. Tinker and Elroy R. Cober
3.0.
Chapter Summary and Objectives 39
3.1. Introduction 40
3.2. Central Concepts in Plant Breeding 41
3.3. Objectives in Plant Breeding 52
3.4. Methods of Plant Breeding 53
3.5. Breeding Enhancements 64
3.6. Conclusions 71 Life Box
3.1. Gurdev Singh Khush 71 Life Box
3.2. P. Stephen Baenziger 73 References 75
4. Plant Development and Physiology 76 Glenda E. Gillaspy and Catherine P. Freed
4.0.
Chapter Summary and Objectives 76
4.1. Plant Anatomy and Morphology 77
4.2. Embryogenesis and Seed Germination 78
4.3. Meristems 84
4.4. Leaf Development 87
4.5. Flower Development 90
4.6. Hormone Physiology and Signal Transduction 93
4.7. Conclusions 99 Life Box
4.1. Natasha Raikhel 99 Life Box
4.2. Brenda S.J. Winkel 101 References 103
5. Tissue Culture: The Manipulation of Plant Development 105 Vinitha Cardoza
5.0.
Chapter Summary and Objectives 105
5.1. Introduction 105
5.2. History of Tissue Culture 106
5.3. Media and Culture Conditions 107
5.4. Sterile Technique 109
5.5. Culture Conditions and Vessels 111
5.6. Culture Types and Their Uses 113
5.7. Regeneration Methods of Plants in Culture 119
5.8. Rooting of Shoots 122
5.9. Acclimation 122
5.10. Automation in Plant Tissue Culture 123
5.11. Artificial Intelligence (AI) and Machine Learning in Plant Tissue Culture 123
5.12. Problems That Can Occur in Tissue Culture 123
5.13. Conclusions 124 Acknowledgments 124 Life Box
5.1. Vinitha Cardoza 125 Life Box
5.2. Raymond D. Shillito 126 References 129
6. Molecular Genetics of Gene Expression 133 Maria Gallo and Alison K. Flynn
6.0.
Chapter Summary and Objectives 133
6.1. The Gene 134
6.2. DNA Packaging into Eukaryotic Chromosomes 134
6.3. Transcription 135
6.4. Translation 144
6.5. Protein Postranslational Modification 150 Life Box
6.1. Maarten Chrispeels 150 Life Box
6.2. Hong S. Moon 152 References 153
7. Plant Systems Biology 155 Wusheng Liu, Yongil Yang, and C. Neal Stewart, Jr.
7.0.
Chapter Summary and Objectives 155
7.1. Introduction 156
7.2. Defining Plant Systems Biology 157
7.3. Properties of Plant Systems 158
7.4. A Framework of Plant Systems Biology 160
7.5. Disciplines and Enabling tools of Plant Systems Biology 162
7.6. Conclusions 179 Life Box
7.1. C. Robin Buell 180 Life Box
7.2. Joshua Yuan 182 References 183
8. Recombinant DNA, Vector Design, and Construction 185 Stephen L. Gasior, David G.J. Mann, and Mark D. Curtis
8.0.
Chapter Summary and Objectives 185
8.1. Plasmids are Unique Genetic Elements in Nature 186
8.2. DNA Vectors 189
8.3. Recombinant DNA Methods 195
8.4. Vector Design in Plant Research and Trait Development 206
8.5. Vectors for Targeted Genome Manipulations 213
8.6. Prospects 216 Life Box
8.1. David Mann 216 References 218
9. Genes and Traits of Interest 224 Joanna H. Kud and Kenneth L. Korth
9.0.
Chapter Summary and Objectives 224
9.1. Introduction 225
9.2. Identifying Genes of Interest Via Omics Technologies 225
9.3. Traits for Improved Crop Production Using Transgenics 228
9.4. Conclusion 245 Life Box
9.1. Tony Shelton 246 References 247
10. Promoters and Marker Genes 249 Wusheng Liu, Debao Huang, C. Neal Stewart, Jr., and Brian Miki
10.0.
Chapter Summary and Objectives 249
10.1. Introduction 250
10.2. Promoters 250
10.3. Marker Genes 259
10.4. Marker- Free Strategies 270
10.5. Conclusions 272 Life Box
10.1. Wusheng Liu 274 Life Box
10.2. Yunde Zhao 275 References 277
11. Transgenic Plant Production 282 John J. Finer and Ning Zhang
11.0.
Chapter Summary and Objectives 282
11.1. Overview of Plant Transformation 283
11.2. Agrobacterium Tumefaciens 286
11.3. Particle Bombardment 293
11.4. Other Methods of Transformation 297
11.5. The Rush to Publish 300
11.6. A Look to the Future 306 Life Box
11.1. John Finer 306 Life Box
11.2. Kan Wang 308 Life Box
11.3. Ted Klein 310 References 312
12. Analysis of Transgenic Plants 315 C. Neal Stewart, Jr.
12.0.
Chapter Summary and Objectives 315
12.1. Essential Elements of Transgenic Plant Analysis 316
12.2. Assays for Transgenicity, Insert Copy Number, and Segregation 317
12.3. Transgene Expression 323
12.4. Knockdown or Knockout Analysis Rather Than Overexpression Analysis 326
12.5. The Relationship Between Molecular Analyses and Phenotype 327 Life Box
12.1. Neal Stewart 327 Life Box
12.2. Nancy A. Reichert 329 References 331
13. Plastid Genetic Engineering 332 Alessandro Occhialini and Scott C. Lenaghan
13.0.
Chapter Summary and Objectives 332
13.1. Introduction 333
13.2. Plastid Biology and Molecular Genetics 334
13.3. Plastid Genetic Engineering History and Motivations 336
13.4. Plastome Engineering Versus Nuclear Genome Engineering 336
13.5. Key Components for Plastome Engineering of Plants 338
13.6. Plastome Transformation Vector Design 340
13.7. Beyond Transplastomics: The Use of Episomal Vectors for Minisynplastome and Minichromosome Approaches 347
13.8. Removing DNA from Plastids 349
13.9. The Future of Plastid Engineering 350 Life Box
13.1. Henry Daniell 351 Life Box
13.2. Pal Maliga 354 Life Box
13.3. Alessandro Occhialini 356 Life Box
13.4. Ralph Bock 357 References 358
14. CRISPR- Cas: Genome Editing from Small- Scale to High Throughput for Plant Biology and Biotechnology 366 S.P. Avinash, Mirza J. Baig, and Kutubuddin A. Molla
14.0.
Chapter Summary and Objectives 366
14.1. Introduction 367
14.2. Diverse CRISPR Approaches and Tools for Precisely Editing Genomes 371
14.3. Changing Gene Expression by CRISPR 374
14.4. CRISPR Screening for Large- Scale Functional Genomics 376
14.5. CRISPR- Enabled Crop Improvement 377
14.6. Commercialized Genome- Edited Crops 380
14.7. Conclusions 380 Life Box
14.1. Kutubuddin Molla 381 Life Box
14.2. Dan Voytas 382 Life Box
14.3. Yiping Qi 383 References 385
15. Regulations and Biosafety 390 Alan Mchughen and Stuart J. Smyth
15.0.
Chapter Summary and Objectives 390
15.1. Introduction 390
15.2. History of Genetic Engineering and its Regulation 392
15.3. Regulation of GE Plants 394
15.4. Regulatory Flaws and Invalid Assumptions 402
15.5. The State of Genome Editing Regulation 406
15.6. Conclusion 409 Life Box
14.1. Alan Mchughen 411 References 412
16. Field Testing of Transgenic Plants: Risk Assessment and Performance 415 Detlef Bartsch, Achim Gathmann, Arti Sinha, and Christiane Saeglitz
16.0.
Chapter Summary and Objectives 415
16.1. Introduction 416
16.2. Environmental Risk Assessment Process 416
16.3. An Example Risk Assessment: The Case of Bt Maize 418
16.4. Proof of Safety Versus Proof of Hazard 422
16.5. Modeling the Risk Effects on a Greater Scale 422
16.6. Proof of Benefits: Agronomic Performance 423
16.7. Conclusions 424 Life Box
18.1. Detlef Bartsch 426 References 427
17. Intellectual Property in Agricultural Biotechnology: Strategies for Open Access 429 Gregory Graff, David Jefferson, Monica Alandete-Saez, Cecilia Chi-Ham, Sara Boettiger, and Alan B. Bennett
17.0.
Chapter Summary and Objectives 429
17.1. Intellectual Property and Agricultural Biotechnology 430
17.2. The Relationship Between Intellectual Property and Agricultural Research 433
17.3. Patenting Plant Biotechnology: The Anti- Commons Problem 434
17.4. What Is Freedom to Operate? 438
17.5. Strategies for Open Access 441
17.6. Conclusions 443 Life Box
17.1. Alan Bennett 444 Life Box
17.2. Maud Hinchee 445 References 446
18. Why Transgenic Plants Are So Controversial 451 Jennifer Trumbo and Douglas Powell
18.0.
Chapter Summary and Objectives 451
18.1. Introduction 452
18.2. Perceptions of Risk 454
18.3. R
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